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In particular, miR-16 was reported to be downregulated in osteosarcoma cell lines and tissues ( in vitro and tumor growth in mice by targeting directly the IGF1R with subsequent inhibition of the Raf1- Mitogen-Activated Protein Kinase Kinase 1/2 (MEK1/2)-ERK1/2 pathway ( Similar results were described for miR-100 also, which expression was significantly reduced in osteosarcoma tissues and cell lines ( A further study showed decreased miR-133a in osteosarcoma cell lines, and this miRNA was shown to control cell proliferation, migration and invasion in vitro , and growth and metastasis in vivo ( in vitro experiments showed that miR-133a overexpression decreased cell proliferation, invasion and migration
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