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Bacterial expression produces none of those and instead produces host-cell protein, host-cell DNA, endotoxin from the Gram- negative cell wall, species with incorrect or scrambled disulfide pairing, N-terminal heterogeneity from incomplete or over-enthusiastic processing of the initiator methionine and the leader region, methionine oxidation, deamidation, clipped forms generated by host proteases, and covalent or non-covalent aggregate