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Both sleep length and quality are influenced by endocrine factors such as somatoliberin (growth hormone-releasing hormone),7 which contributes to slow-wave sleep8 — an indicator of healthy slumber.9 Some research suggests, though not conclusively, that DSIP can stimulate somatoliberin secretion,10 thereby promoting the onset of a deep stage of sleep

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The membranes were blocked with 5% (w/v) skimmed milk in TBS containing 0.1% (v/v) Tween 20 (blocking solution) for 1 h and incubated with the rabbit anti-MUC5B polyclonal antibody LUM5B-2 67 , which is directed against the MUC5B CYS domain (12 copies of which were present in the Tg product)