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[DOI] [PMC free article] [PubMed] [Google Scholar] 35.Cahill P.A., Redmond E.M

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Gene expression levels were determined by qRT-PCR in the Quantifast SYBR Green PCR mix (Qiagen GmbH, Germany) with a reaction condition of initial denaturation at 95 °C for 5 min and then 40 cycles of 95 °C for 10 s and 60 °C for 30 s in QuantStudio 3 machine