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In this context, the recently developed programmable Clustered Regularly Interspaced Short Palindromic Repeats interference (CRISPRi) system has proved to be an effective tool to interrogate essential genes in bacteria including mycobacteria (Agarwal, 2020

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The levels of formation of N 2 -(ß-1-glucosiduronyl-2-(hydroxyamino)-1-methyl-6-phenylimidazo[4,5- b ]pyridine (HON-PhIP- N 2 -Gl), the principal metabolite of PhIP excreted in human urine (98,101,103,176) , showed a high interindividual variability in formation, up to 28-fold, with human liver microsomes (398)