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Notably, LASSS displayed additional functions to promote receptor binding-affinity and nitration resistance of HGF in a dose-dependent manner, that is high-dose LASSS in excess of a 1:4000 molar ratio to HGF, as revealed by biochemical assays after wash-out of un-reacted free LASSS (Figure 2a-d)
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NDMA metabolism generates highly reactive methylating metabolites (e.g., O6-methylguanine, m6G), which bind to DNA, resulting in base mutations and an increased risk of bladder cancer (Anderson et al., 1996)
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