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Future studies must prioritize tumor-specific delivery systems and the use of biomarkers such as ACSL4 and GPX4 for patient stratification to translate these findings into clinical practice
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In most cases, where the fusion protein is initially observed primarily in inclusion bodies, it is desirable to modify culture conditions—such as lowering growth temperature, increasing aeration, or altering induction conditions to—attempt to obtain soluble protein, rather than to solubilize inclusion bodies with denaturants and refold the protein after extraction
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