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For preparation of samples for SDS-PAGE, clarified lysates were adjusted to have equal protein concentrations across samples and then mixed with 4X Laemmli sample buffer containing 10% (v/v) 2-mercaptoethanol to achieve a final 1X concentration and then heated at 72°C for 10 min

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Gene Ontology (GO) terms distribution for the molecular functions (Supplementary Figure S1C) of all the regulated proteins from clusters 1, 3, and 4 showed annotation to catalytic activity, binding proteins whereas cluster 1 represented a high amount of protein enrichment for RNA binding and structural molecule activity