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Proteins were then precipitated by the addition of the total sample volume to 4× volume liquid chromatography–mass spectrometry (LC–MS)-grade acetonitrile, as per the SP4 protocol 63 , followed by gentle shaking for 5 min and centrifugation at 3,220 g for 10 min
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10.4168/aair.2021.13.3.450 [DOI] [PMC free article] [PubMed] [Google Scholar] Wochner R
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