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The method is based on the ability of silver ions to form weak charge-transfer complexes with the olefinic double bonds and the fact that cis -unsaturated fatty acids form stronger complexes than the trans geometric isomers
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Recording with a camera attached to a VMS-004 Discovery Deluxe USB microscope (Veho, Claymont, DE, USA) was performed until the end of the experiment and assessed at 60 min (25 mmHg), 30 min (30 mm Hg), or 25 min (40 mm Hg) reperfusion time
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